RESUMEN
The aim of the present study was to investigate the in vitro antioxidant and antitumor effects of Salvia fruticosa Mill subsp. thomasii (Lacaita) Brullo, Guglielmo, Pavone & Terrasi (Lamiaceae). The aerial parts were extracted by maceration with methanol. This extract was partitioned with methanol and n-hexane. Luteolin, luteolin 7-O-glucoside, rutin and salvigenin were isolated from the methanol-soluble fraction. n-Hexane fraction showed viridiflorol, ß-pinene, 1,8-cineole, as main components. The methanol-soluble fraction exerted antitumor activity against human breast cancer (MCF-7 and MDA-MB-231) and human colorectal carcinoma (RKO and Caco-2) cells. TUNEL test revealed that S. fruticosa subsp. thomasii leads to cells death by apoptosis, with low cytotoxic effects on non-tumoral 3T3-L1 cells. Moreover, it exerted the highest protection of lipid peroxidation and reduced the oxidative stress induced by menadione treatment in 3T3-L1 murine fibroblasts. S. fruticosa subsp. thomasii bioactivity could promote its use not only as food but also in nutraceutical/pharmaceutical industries.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Antioxidantes/farmacología , Extractos Vegetales/farmacología , Salvia/química , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Humanos , Componentes Aéreos de las Plantas/química , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificaciónRESUMEN
A series of research on plants used in Calabria (Southern Italy) in the folk plant medicine was carried out in the last twenty years. The use of 104 taxa distributed into 42 families are described. Among the major findings: Ceterach officinarum as hypotensive; Heliotropium europaeum in the case of urinary inflammations; Silybum marianum for haemorrhoids; Arundo donax for pertussis and cystitis; Allium cepa for sties and flu; Laurus nobilis as galactagogue; Passiflora incarnata for burns, haemorrhoids and skin inflammations; Scrophularia canina for rhagas and wounds; Urtica membranacea for tussis and tonsillitis.
Asunto(s)
Medicina Tradicional , Fitoterapia , Preparaciones de Plantas/uso terapéutico , Plantas Medicinales , Etnobotánica , Humanos , ItaliaRESUMEN
The present clinical study was performed to comparatively assess the therapeutic effect of Low and High Molecular Weight Chitosan (LMWCh and HMWCh), hexetidine, triclosan. Plaque index, saliva buffering capacity and bacteriological controls for S. mutans and lactobacilli, were performed. The plaque and bacterial indices revealed statistically significant differences between groups. Buffering capacity was similar using, hexetidine, and triclosan, whereas it was maximum in 100% of the patients in the LMWCh and HMWCh groups. Only 0.5% HMWCh induced low activity of S. mutans in 100% of the patients and caused complete inhibition of lactobacilli growth. No changes were observed in the profile of salivary proteins. The present clinical study confirms the therapeutic efficacy of the chitosan as a bacteriostatic agent.
Asunto(s)
Antiinfecciosos Locales/administración & dosificación , Quitina/análogos & derivados , Quitina/administración & dosificación , Placa Dental/tratamiento farmacológico , Hexetidina/administración & dosificación , Triclosán/administración & dosificación , Adolescente , Adulto , Quitosano , Recuento de Colonia Microbiana , Placa Dental/microbiología , Método Doble Ciego , Femenino , Humanos , Lactobacillus/efectos de los fármacos , Masculino , Peso Molecular , Antisépticos Bucales/farmacología , Antisépticos Bucales/uso terapéutico , Saliva/microbiología , Streptococcus mutans/efectos de los fármacosRESUMEN
The present clinical study was performed to comparatively assess the therapeutic effect of Low and High Molecular Weight Chitosan (LMWCh and HMWCh), hexetidine, triclosan. Plaque index, saliva buffering capacity and bacteriological controls for S. mutans and lactobacilli, were performed. The plaque and bacterial indices revealed statistically significant differences between groups. Buffering capacity was similar using, hexetidine, and triclosan, whereas it was maximum in 100
of the patients in the LMWCh and HMWCh groups. Only 0.5
HMWCh induced low activity of S. mutans in 100
of the patients and caused complete inhibition of lactobacilli growth. No changes were observed in the profile of salivary proteins. The present clinical study confirms the therapeutic efficacy of the chitosan as a bacteriostatic agent.
RESUMEN
The present clinical study was performed to comparatively assess the therapeutic effect of Low and High Molecular Weight Chitosan (LMWCh and HMWCh), hexetidine, triclosan. Plaque index, saliva buffering capacity and bacteriological controls for S. mutans and lactobacilli, were performed. The plaque and bacterial indices revealed statistically significant differences between groups. Buffering capacity was similar using, hexetidine, and triclosan, whereas it was maximum in 100
of the patients in the LMWCh and HMWCh groups. Only 0.5
HMWCh induced low activity of S. mutans in 100
of the patients and caused complete inhibition of lactobacilli growth. No changes were observed in the profile of salivary proteins. The present clinical study confirms the therapeutic efficacy of the chitosan as a bacteriostatic agent.
